Alei Geng, Ph.D., Lecturer
2011, Ph.D., Biochemistry and Molecular Biology,
2005, B.S., Bioengineering,
Main Research Interests:
Mechanism study in biological pretreatment of lignocellulose by termite;
Enzyme engineering of cellulases and biological pretreatment enzymes.
Contribution to Science and Technology:
1. An oxidase purified from the tissues of Coptotermes formosanus was characterized and found to have potential application in biological pretreatment of lignocelluloses.
2. A metagenomic fosmid library was construct to screen for cellulase genes which presented in a biogas digester of rice straw, and 12 novel cellulase genes were obtained. An efficient neutral endoglucanase Exo2b was characterized and found to have the potential application in improving the performance of Trichoderma reesei on lignocellulose hydrolysis.
3. Anaerobic biohydrogen production from cellulose was carried out by a co-culture of a cellulolytic, hydrogen-producing bacterium (Clostridium thermocellum DSM 1237) and a non-cellulolytic, hydrogen-producing bacterium (Clostridium thermopalmarium DSM 5974). The effects of the inoculation ratio, yeast extract concentration, and alkalinity on the co-culture system were investigated, and the dynamics of the abundance of the two strains during co-culture were monitored using Real-time PCR.
Alei Geng, Gen Zou, Xing Yan, Qianfu Wang, Jun Zhang, Fanghua Liu, Baoli Zhu, Zhihua Zhou. 2012. Expression and characterization of a novel metagenome-derived cellulase Exo2b and its application to improve cellulase activity in Trichoderma reesei. Applied Microbiology and Biotechnology, 96: 951-962. IF: 3.4
Alei Geng, Yanling He, Changli Qian，Xing Yan and Zhihua Zhou. 2010. Effect of Key Factors on Hydrogen Production from Cellulose in a Co-Culture of Clostridium thermocellum and C. thermopalmarium. Bioresource Technology, 101: 4029–4033. IF: 4.9
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